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Five-week-old BALB/c female nude mice were purchased from Shanghai Laboratory Animal Center, Chinese Academy of Sciences and Technology (Shanghai, China) and used for this experiment. Animals were housed and maintained in specific pathogen-free conditions according to the recommendation of Guide for the Care and Use of Laboratory Animals of the National Institutes of Health with strict adherence to protocols approved by the Institutional Animal Care and Use Committee of Guizhou Medical University. Mice were injected subcutaneously with 2 × 106 cells in 200 uL of serum-free DMEM medium. Tumor size (length and width, mm) was measured with an vernier caliper every other day and tumor volumes (mm^3^) were calculated as 0.5 × length × width^2^. Animals were sacrificed on the 27th day after inoculation for histopathologic analysis and the remaining were sacrificed at the end of the experiment.
Mice were injected subcutaneously with 1 × 106 cells in 200 uL of serum-free DMEM medium. The tumor size (length and width, mm) was measured with an vernier caliper every other day and tumor volumes (mm^3^) were calculated as 0.5 × length × width^2^. Animals were sacrificed on the 27th day after inoculation for histopathologic analysis and the remaining were sacrificed at the end of the experiment. Tumor tissues were fixed in 10% formalin solution and paraffin embedded for further experiments.
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