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In terms of function, the novel discovery of C. deuterogattii EV-based long-distance pathogen-to-pathogen communication (which has not been reported for non-gattii strains) [81], its role in exploiting host immune cells, and ultimate impact on virulence [79,81] provide strong evidence that the functional mechanism of EVs differs in the two lineages. This discovery and other findings on CGSC-specific EV traits highlight the need for probing the already-studied concepts of C. neoformans-derived EV and novel EV-associated phenotypes in these other species. Research focused on CGSC EVs will not only diversify our understanding of cryptococcal EVs but also holds potential for revealing new paradigms around the biological and pathogenic functions of fungal EVs more broadly. 2b1af7f3a8